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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in many tumors, which leads to chromosome instability, and thus may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical position in marketing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two supplemental splice variants had been reported but in-depth nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide generally is made up of the epitope regarded through the antibody.
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This information outlines additional doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make sure the absolute best final results are...
Antibodies certain to the blocking peptide not bind into the epitope about the concentrate on protein. By evaluating the staining within the blocked antibody versus the antibody alone, you can read more see which staining is precise.
Focus on Data Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and capabilities as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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